This book contains the methods of collection, labeling, storage and transportation of samples; biochemical tests like serum glucose, protein, lipid, BUN, creatinine, urea, uric acid etc.; kidney function test; liver function test; pancreatic function test, microbiological and parasitological tests etc. Various laboratory methods for estimating different blood constituents and urine along with the interpretation are given so that it is easier for the beginner to understand the subject at ease. The book covers all the basic and essential information on veterinary laboratory diagnosis needed for B.V.Sc. & A.H students, Master degree students, teachers and veterinary clinicians. The book will also be a helpful aid for the students preparing ICAR-JRF, ICAR-NET, ICAR-SRF, PhD and different competitions related to veterinary discipline. This book is an attempt to suit the updated course curriculum as per VCI regulations. We hope this textbook will fulfill the requirement of the veterinary students in the field of veterinary laboratory diagnosis.