The generation of waste in most industrial processes is practically unavoidable. In the oil industry, one of the major problems for the environment is the large volume of water produced in oil production processes. Currently, the use of flotation has been very efficient compared to other methods, as they are unable to remove much of the oil that is emulsified. In this context, this work aims to evaluate the efficiency of column flotation processes using biosurfactant as a demulsifying agent that accelerates phase separation.